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Introduction of microbiological diagnostic agents of invasive infections - Bartonella henselae and Bartonella quintana
Nováková, Marie ; Melter, Oto (advisor) ; Chrenková, Vanda (referee)
Bartonellae are agents of infections with varied clinical symptomalogy, including serious, such as endocarditis. Direct detection of Bartonella so far in the Czech Republic and therefore does not need to address this issue. Proper diagnosis of these infections is a prerequisite for targeted antibiotic therapy for patients. The genus Bartonella today are more than 20 kinds of bacteria. They are small Gram-negative rod-shaped bacteria that cause the new emerging infections such as cat scratch disease (CSD), showing the typical regional lymphadenitis, as well as bacillary angiomatosis (BA), whose typical symptoms are skin and subcutaneous nodules (nodules). They also cause a disease called trench fever or South American bartonelosis. In the experimental part of the work was performed genotypic and phenotypic methods for the diagnosis of these bacteria, specifically on two types of Bartonella, Bartonella henselae and Bartonella quintana . The results show that by suitable methods to diagnose Bartonella are amplification genus- specific genes ribC and ftsZ, further embodiment of a method pulsed electrophoresis (PFGE) as typing method and the determination of susceptibility to antibiotics, the most appropriate testing showed E-test. This work was carried out optimization of genotypic and phenotypic...
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Introduction of microbiological diagnostic agents of invasive infections - Bartonella henselae and Bartonella quintana
Nováková, Marie ; Melter, Oto (advisor) ; Chrenková, Vanda (referee)
Bartonellae are agents of infections with varied clinical symptomalogy, including serious, such as endocarditis. Direct detection of Bartonella so far in the Czech Republic and therefore does not need to address this issue. Proper diagnosis of these infections is a prerequisite for targeted antibiotic therapy for patients. The genus Bartonella today are more than 20 kinds of bacteria. They are small Gram-negative rod-shaped bacteria that cause the new emerging infections such as cat scratch disease (CSD), showing the typical regional lymphadenitis, as well as bacillary angiomatosis (BA), whose typical symptoms are skin and subcutaneous nodules (nodules). They also cause a disease called trench fever or South American bartonelosis. In the experimental part of the work was performed genotypic and phenotypic methods for the diagnosis of these bacteria, specifically on two types of Bartonella, Bartonella henselae and Bartonella quintana . The results show that by suitable methods to diagnose Bartonella are amplification genus- specific genes ribC and ftsZ, further embodiment of a method pulsed electrophoresis (PFGE) as typing method and the determination of susceptibility to antibiotics, the most appropriate testing showed E-test. This work was carried out optimization of genotypic and phenotypic...
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